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CABRI:DSMZ_MUTZ |
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Accession_number |
ACC 173
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Cell_line_name |
3T3
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Brief_description |
confirmed as mouse with IEF of AST, LDH, PEP B established from disaggregated
Swiss albino mouse embryos in 1962; cells are used for transfection studies with DNA
viruses and as assay system for transformation studies; cells have a high sensitivity to
contact inhibition
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Description |
Swiss albino mouse fibroblasts established from disaggregated Swiss albino mouse
embryos in 1962; cells are used for transfection studies with DNA viruses and as
assay system for transformation studies; cells have a high sensitivity to contact
inhibition confirmed as mouse with IEF of AST, LDH, PEP B
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Depositor |
Dr. J. Wehland, GBF, Braunschweig, Germany
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Morphology |
fibroblasts growing adherently as monolayer with contact inhibition
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Culture_conditions |
90-95% Dulbecco's MEM + 5-10% FBS split confluent culture at a ratio of up to 1:8
every 2 to 4 days using trypsin/EDTA; after trypsination cells might remain in
aggregates that are difficult to disperse; upon reaching confluence cells stop to grow;
cells grow fast at low titer; seed out at ca. 1-2 x 106 cells/175 cm 2 at 37C with 5%
CO2 cell harvest of about 5-8 x 106 cells/175 cm 2
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Viruses |
ELISA: reverse transcriptase negative; PCR: SMRV -
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Properties |
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Release_conditions |
-
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Hazard |
-
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Storage |
frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 106 cells/ampoule
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Doubling_time |
ca. 40 hours
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Mycoplasma |
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Cytogenetics murine hypertriploid (near tetraploid sideline present) karyotype with 13% polyploidy -
68(58-73)<3n>
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Bibliographic_reference |
Todaro et al., J Cell Biol, 17: 25 (1963) [PMID: 13985244
] |
Todaro et al., Virology, 27: 25 (1965) [PMID: 4284655]
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